The video for "Dude (Looks Like a Lady)" features the band performing live onstage as well as random moments of characters portraying transvestites, including a cameo appearance by A&R man John Kalodner dressed up in a wedding dress at one point. This is a joke based on the fact that Kalodner always dresses in white.
There are also some provocative sexual performances, both lead by singer Steven Tyler as well as a presumed female who has her skirt torn off to reveal the Aerosmith "wings" tattoo on her buttocks. The video was directed by Marty Callner.
In 1993, the song was prominently featured in the film Mrs. Doubtfire, during a montage of the main character bonding with his family in disguise as a woman.